rabbit anti mouse perilipin  (Novus Biologicals)

Journal: EXCLI Journal

Article Title: Long-term Western diet feeding impairs hepatic vitamin D metabolism and promotes bone loss in mice

doi: 10.17179/excli2025-9085

Figure Lengend Snippet: Histological assessment of bone composition. (A) Representative image of perilipin-positive adipocytes (arrowheads) from SD and WD mice at week 48. Scale bars: 20 µm. The average nuclear area was 14.67 µm² in SD mice and 14.22 µm² in WD mice. (B) Quantitative analysis of perilipin-positive adipocytes from the two groups. (C) Representative image of CD163-positive macrophage (arrowheads) from SD and WD mice at week 48. (D) Quantitative analysis of CD163-positive macrophages from the two groups. Scale bars: 20 µm. The average nuclear area was 14.43 µm² in SD mice and 14.50 µm² in WD mice. Data are shown as box-and-whisker plots. Statistical analysis was performed using the Mann-Whitney U test. * p < 0.05, ** p < 0.01 as compared to SD group. SD = standard diet, WD = Western diet.

Article Snippet: For immunohistochemical staining, sections were incubated for 1 h with a monoclonal rabbit anti-mouse antibody against perilipin (1:200; Cell Signaling Technology, Danvers, MA, USA) and a rabbit anti-CD163 (1:300; Abcam, Cambridge, UK) as the primary antibody.

Techniques: Whisker Assay, MANN-WHITNEY, Western Blot

Journal: iScience

Article Title: A simplified and defined serum-free medium for cultivating fat across species

doi: 10.1016/j.isci.2022.105822

Figure Lengend Snippet: Cells differentiated with rDM present high expression of adipogenic markers at the gene and protein level (A) Mean gene expression fold changes 12 days after induction of bovine SVC differentiation, determined by qPCR. Adiponectin ( ADIPOQ ), cell death activator ( CIDEC ), and stearoyl-CoA desaturase ( SCD ). All conditions were normalized to a chosen set of reference genes ( UXT, RPLP, L19 ) and to day 0 control (not shown); ∗mean 2 - ΔΔ Ct values of each condition were divided by the average of same isolation to improve comparability between conditions. Data are represented as mean ± SD; the error bars represent the SD of 4 independent experiments using 4 donors. One-way ANOVA; NS, not significant; ∗p < 0.05; ∗∗∗p < 0.001; and ∗∗∗∗p < 0.0001. All conditions are compared to rDM. (B) Immunohistochemical analysis of bovine SVC differentiated in rDM for 8 days. PPARγ and CEBPA are early differentiation markers; Acetyl-CoA carboxylase 1 (ACACA) and Perilipin 1 (PLIN1) are maturation markers. Scale bar, 100 μm. See also Figures S3 and .

Article Snippet: anti-mouse PLIN1 , Cell signalling technology , Rabbit mAb Cat #9349; RRID: AB_10829911.

Techniques: Expressing, Gene Expression, Control, Isolation, Immunohistochemical staining

Journal: iScience

Article Title: A simplified and defined serum-free medium for cultivating fat across species

doi: 10.1016/j.isci.2022.105822

Figure Lengend Snippet: rDM outperforms cDM in both 3%FBS and DMAD during long-term (28-day) 3D culture (A) Illustration of the experimental design. Bovine SVC were encapsulated with alginate hydrogel and differentiated with 3%FBS or DMAD. cDM contained all 4 inducers during first media change followed by progression for the whole period of culture, rDM contained rosiglitazone and insulin for 28 days. DMAD contained HC/PR. (B) Representative images of maximum intensity projection confocal microscopy. Differentiation with control (cDM) or reduced (rDM) differentiation medium in 3%FBS or DMAD at day 28. Blue, Hoechst; and yellow, Nile Red. (C) PLIN1 immunohistochemistry of 3D hydrogel/cell constructs differentiated in cDM or rDM medium at day 28. Blue, Hoechst; red, PLIN1; and yellow, Nile red. (B and C) Scale bar, 100 μm. (D) Quantitative analysis of glycerol release at day 0, 7 and 28. Data is representative of 3 independent experiments using 4 donors. (E) Qualitative and quantitative assessment of the amount of fatty acids within triglycerides (normalized to the amount of DNA). Results are representative of 5 independent experiments using 6 donors. (D and E) Statistical differences were analyzed with a one-way ANOVA. Data are represented as mean ± SD; NS, not significant; ∗∗∗p < 0.001. See also Figure S5 and .

Article Snippet: anti-mouse PLIN1 , Cell signalling technology , Rabbit mAb Cat #9349; RRID: AB_10829911.

Techniques: Confocal Microscopy, Control, Immunohistochemistry, Construct

Journal: iScience

Article Title: A simplified and defined serum-free medium for cultivating fat across species

doi: 10.1016/j.isci.2022.105822

Figure Lengend Snippet:

Article Snippet: anti-mouse PLIN1 , Cell signalling technology , Rabbit mAb Cat #9349; RRID: AB_10829911.

Techniques: Recombinant, SYBR Green Assay, cDNA Synthesis, RNA Sequencing, Software